Next-Generation Sequencing (NGS)

Overview

Sequencing primers are short oligonucleotides that initiate DNA synthesis during sequencing reactions for the application of next-generation sequencing (NGS).

Prior to sequencing, genomic libraries are prepared by attaching adapter sequences to the ends of DNA fragments to allow for binding of the libraries to the sequencing platform, binding sites for sequencing primers and even multiplexing for sample identification. NGS primer and adapter sequences must be carefully designed and optimized to reduce off-target annealing and amplification, and any added chemical modifications must be adequately removed prior to use in downstream applications.

Oligo Factory leverages finely tuned solid phase synthetic chemistry to produce high-quality NGS primers and adapters from microgram to hundreds of grams quantities. Our NANDA Series synthesizers enable precise control and enhanced purity for even the most complex probe and primer sequences with optimized GC content and balanced adapter efficiency and bias in NGS applications.