Gene Expression Modulation

Overview

ASOs are short, synthetic single-stranded DNA or RNA molecules designed to selectively bind to messenger RNA (mRNA) and modulate gene expression.

This binding can either block the translation of the mRNA into protein or trigger its degradation, effectively “silencing” the target gene. This mechanism of action makes ASOs invaluable in treating a wide range of diseases, from rare genetic disorders to cancers, and must be manufactured with high purity and accuracy to ensure specificity. ASOs may also require the addition of complex modifications to enhance binding and stability for improved efficacy.

Gapmers are specialized ASOs designed with a central DNA “gap” flanked by chemically modified nucleotides. This unique structure enhances target binding affinity, nuclease resistance, and RNase H recruitment, making gapmers powerful tools for gene silencing and modulation. As the demand for precise gene therapies grows, high-quality gapmer production becomes increasingly critical for therapeutic development and clinical applications.  

Oligo Factory leverages finely tuned solid phase synthetic chemistry to produce high-quality ASOs and Gapmers from microgram to hundreds of grams quantities. Our NANDA Series synthesizers enable precise control and enhanced purity for even the most complex ASO sequences.