RUO Oligos
Specializing in the synthesis of
custom DNA and RNA oligonucleotides
Custom RUO Oligos
Since 2006, Oligo Factory has specialized in producing custom RUO-quality DNA and RNA oligos at scale.
Our solid-phase synthesis approach provides outstanding quality and purity for oligos with 200 bases or fewer. With our 100% US-based primary supply chain, we have shipped in six weeks or less every single order since January 2022.
Discover how Oligo Factory’s approach delivers high-quality, custom dual dye labelled DNA probes with rapid turnaround times while maintaining cost efficiency in this case study.
COMMON OLIGO TYPES
What We Build
Our team uses in-house-developed synthesizers to produce your oligos faster and with higher initial purity, even for complex sequences with poly-base strings or complex modifications. We also specialize in modified DNA and RNA, from probes to spacers to inverted bases.
Oligo Factory specializes in working with unusual modifications and post-synthesis conjugations. Some of our most commonly-ordered modifications include:
- 2′OMe, 2′MOE and 2′F RNAs
- Full or partial phosphorothioate linkages
- C3-Amino, C6-Amino, C12-Amino
- C-Spacers and PEG
- DBCO and other linkers
- FAM, ROX, Cy3, Cy5, BHQ, Alexa, and other fluorescent markers
- BHQ, iQuench, and other quenchers
- GalNAc and other cholesterols
- LNAs and BNAs
- C3-Thiol, C6-Thiol
- Inverted nucleotides
- 5′-phosphate
- 5′-Biotin, 3′-Biotin, dT-Biotin
- TBDMS, TOM and PivOM phosphoramidites
- Post-synthesis modifications
Additional Modifications
Interested in a modification that you don’t see listed here? Talk to a scientist to find out how we can jump-start your project or scale up your existing oligo!
Synthesis & Purification
Proprietary Synthesis Technology
Our third-generation in-house built solid-phase oligo synthesizer runs twice as fast as commercially-available synthesizers, provides outstanding initial purity, and is designed to work most efficiently at scale.
Purification
Our purification team uses several industry approved purifications via HPLC – both reverse-phase and ion-exchange. We also can develop in-house options per customer requirement to maximize yield and purity needs. Purity specifications are set by the customer, but we routinely manufacture to a purity of 90%+, 95%+, or higher as needed.
Consultation & Process Development
Our scientists are here to assist from start to finish with your oligo synthesis project. We routinely assist our customers and partners with synthesis and purification protocol design and transfer as well as any analytical needs you may have.
To speak to one of our scientists or get a quote for your oligo synthesis project, please click the link below.
Address:
56 Boynton Rd, Holliston, MA 01746
Contact:
Phone: (508) 275-3561
Email: Info@oligofactory.com
