Guide RNA

Precision tools for CRISPR-based genome editing

OVERVIEW

Advancing CRISPR Technology

Guide RNA (gRNA or sgRNA) is a keystone component in CRISPR-Cas9 gene editing systems. As short oligonucleotides, gRNAs are responsible for directing the Cas9 enzyme to specific DNA sequences for targeted gene knockouts, insertions, replacements, base editing, and epigenetic modulation. As CRISPR applications in gene therapy, disease modeling, and precision medicine expand, high-quality gRNA production becomes increasingly important to optimize stability and delivery in complex biological systems and minimize off-target edits.

Oligo Factory leverages proprietary oligonucleotide synthesis technology and extensive experience to produce high-quality, scalable sgRNA solutions. Our advanced NANDA Series synthesizers enable precise control and enhanced purity for even the most complex gRNA sequences.

Benefits

Providing Precision Gene Editing Tools

At Oligo Factory, we specialize in synthesizing high-quality gRNAs, customized to your specifications.

Proprietary synthesis technology: Our NANDA Series synthesizers are optimized to produce gRNAs faster at scale and higher purity than other commercial systems.
Custom Synthesis: We specialize in producing guide RNAs tailored to your specific CRISPR applications, including short guide RNAs and longer (100+ bp), more complex sequences.
Quality Assurance: Our reverse-phase and ion-exchange HPLC purification processes routinely achieve high purity levels (based on customer specifications), verified through HPLC analysis and LC-MS molecular weight confirmation.
Regulatory Compliance: Oligo Factory provides comprehensive documentation and product traceability, is ISO 9001:2015 and ISO 13485:2016 certified, and complies with ICH Q7 GMP for APIs and FDA Title 21 CFR 820.

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