PCR

Overview

Primers are short oligonucleotides that serve as starting points for DNA synthesis, enabling the amplification of genetic material in techniques like polymerase chain reaction (PCR).

In addition to amplification, quantitative PCR (qPCR) utilizes DNA probes labeled with fluorescent dyes to measure the amount of DNA present in real-time. Primer and probe sequences must be carefully designed and optimized to reduce off-target annealing and amplification, and any added chemical modifications must be adequately removed prior to use in downstream applications.

Oligo Factory leverages finely tuned solid phase synthetic chemistry to produce high-quality PCR and qPCR primers and probes from microgram to hundreds of grams quantities. Our NANDA Series synthesizers enable precise control and enhanced purity for even the most complex probe and primer sequences with optimized melting temperatures and GC content and minimized primer-dimer formation.

Discover how Oligo Factory’s approach delivers high-quality, custom dual dye labelled DNA probes with rapid turnaround times while maintaining cost efficiency in this case study.