Technology
High Purity Custom Oligos
In House Expertise & Technology to Meet Your Needs
Custom Oligos
In House Expertise
In House Technology
Overview
Delivering high purity custom oligos to meet your specifications
Purification is an essential step in synthetic oligonucleotide manufacturing to ensure maximum yield and purity levels. Our dedicated team of chromatographers and process chemists deploy high pressure liquid chromatography (HPLC) to achieve high levels of purity and quantity. Providing timely, scientist-to-scientist support with our clients from planning through delivery at microgram to hundreds of grams quantities is a key element of our offering and customer-first mindset at Oligo Factory.
Technology
In House State of the Technology to Enable High Purity Custom Oligos
Purification is an essential step in synthetic oligonucleotide manufacturing to ensure maximum yield and purity levels. We primarily deploy the industry standard high pressure liquid chromatography (HPLC) to achieve high levels of purity and quantity.
Anion Exchange HPLC
The method of choice when separation by charge provides maximum separation, such as unmodified oligos. Large amounts of oligonucleotides with non-complex structures are easily purified by this method.
Reverse Phase HPLC
The method of choice when separation can be achieved by the relative hydrophobicity of the oligo. Synthetic oligonucleotides with the DMT group or modified oligonucleotides with dyes/biotin/cholesterol can be purified easily using this technique.
Desalting
The “quick and easy” method for separating oligos as quickly as possible. While desalting does not provide the same level of purity as HPLC, it is available as an option when quicker turn-around time is requested or higher purity is not required.
